The humification of peat deposits has been reported as a useful proxy to indicate paleoclimatic changes, but there is no reports focusing on lacustrine sediments. On the basis of analyses of the humification of lacustrine sediment in Lake Barkol, northeastern Xinjiang, and by comparing with results of multi-proxies such as OM, TOC, carbon and oxygen isotopes of carbonate, we considered that higher humification of lacustrine sediment value indicates relatively humid climate, whereas lower humification of lacustrine sediment indicates relatively dry condition. Based on humification proxy and other proxies, the paleoclimate of Lake Barkol could be divided initially into five phases:early Holocene (9.4-7.5cal kaBP), climate is relative cold and dry;mid-Holocene (7.5-5.8cal kaBP), relatively warm and humid climate prevailed, and then climate turned into warm and dry during 5.8-3.3cal kaBP;in late Holocene, climate became cold and dry;in 1.0-0.0cal kaBP from previous relatively cold and wet period lasting from 3.3 to 1.0cal kaBP.