The hydrophyte has an important effect on removing nitrogen in the wetland system. This paper studied the nitrogen removal rate of the simulated wetland systems with Phragmites communis, Scirpus tabernaemontani cv.Zebrinus. and Vallisneria spiralis L. respectively. The hydrophytes had changed the redox condition markedly, however no significant discrepancies were found on the nitrogen removal rate. For the low nitrogen load (2.85mg/L for NH₄⁺_-N and 1.07mg/L for NO₃^-_-N), the ten-day removal rate of Ammonium and Nitrate of the wetland systems with and without hydrophytes were 39.32% and 21.91%, 34.68% and 13.86% respectively. In contrast, for the high nitrogen load (5.50mg/L for NH₄⁺_-N, and 3.37mg/L for NO₃^-_-N), the ten-day removal rate of Ammonium and Nitrate of the wetland systems with and without hydrophytes were 66.87% and 78.92%, 40.62% and 31.77% respectively. The hydrophyte could promote the process of nitrous transfer and shorten the time of the nitrous stay in the wetland.