The vast propagation of Hydrilla verticillata was studied based on the tissue culture technique. After aseptic system wasestablished, half and full strength of Murashige and Skoog-based liquid media(MS), in addition with 0.5-2 mg/L 6-Benzylaminopurine(BA) and 0.1-0.2 mg/L indoleacetic acid (IAA), were used for shoot induction. Meanwhile, quarter, half and full strength of MS plus0.1-0.2 mg/L IAA were useded for root induction. The results showed that the combination of full strength of MS plus 1 mg/L BAand 0.1 mg/L IAA was the best medium for shoot induction because the plants on such combined condition had more shoot numberand shoot biomass than those in the other mixtures. Half strength of MS plus 0.1 mg/L IAA or full strength MS plus 0.2 mg/L IAAwas the most proper media for root induction, and they can greatly promote the development of roots. The propagation was greatlyenhanced with the shoot induction in the optimal environment, amounting to 1800 times per quarter. The root induction made theseshoots grow into whole plants quickly, which enhanced the colonization ability of propagules at new habitat. In the outdoorexperiment, all plants survived at two densities (100 plants/m², 320 plants/m²) on three sediment types (sand, clay, sand+ clay1. 1V/V)). After 21 days, the population coverage was more than 70%. The experiments demonstrated that tissue culture was an effectivemethod of propagation of tool species to get the vast number of seedlings, and ensures the quality of the plants, and finally establishesa key basis for restoration engineering of submerged vegetation.