Parents fish of Taking fugu obscurus were collected for artificial reproduction in the lower reaches of the Yangtze River from 1995 to 1997. The sexual gland of female puffer can develop to the end of IV period through treating with LHRH-A. The female puffers which the sexual glands have developed to the middle of W period could be induced spawning through in jecting hormone. The hormone for inducing spawn of the experiments is LRH-A、HCG and PG. At the temperature of 16-21 ，the time of effect is 6-86 hours, the average rate of spawning is 84.0%, the aver-age rate of fertilizing is 61. 8%, the average rate of hatching is 51.7%.At the water temperature of 20C°, the time of embryonic development is 154 hours.