Isolates of Microcystis blooms collected from Dianchi Lake of South-western China in different seasons were tested by mouse bioassays. The LD₅₀ of the vcdcrocystis isolates corre-sponding M. wesenbergii, M.viridis and M. aeruginosa were 221 mg · kg ^-1, 188 mg · kg ^-1, and 198 mg · kg ^-1 respectively. The cells of the three samples contained hepatotoxins or microcystins. The HPLC (high performance liquid chromatography) analysis showed that all the three Microcystis species contained microcystin LR. In addition, M. wesenbergii contained microcystin RR, M. viridis contained microcystinYR, M. aeruginosa contained both microcystin RR and YR. The results suggested that in different seasons the toxins which were produced by the dominating Microcystis species were fluctuent with regard to the composition and content of the toxins and consen-quently were obvious factors that aroused the water quality to decrease and change.