This paper studies the effects of DO, pH, temperature and the growth of bacteria on the release of phosphorus from Wuli Lake sediment.The result of experiments shows that the quantity of phosphours released from sediments into the water body was getting greater with decrease in DO, pH, temperature but increase in the growth of bacteria.The filtrate of the anaerobic release test may stimulate the growth of Scendestnus obliquus, but not the growth of Selenaslrum capricornutum.It is showed that filtrate of the aerobic release test can stimulate neither of the algae.