Abstract:This paper deals with the effects of different collecting methods and various storage conditions on hatching rate of brine shrimp (Artemia spp.) cysts from Barkol Lake (43.6°N, 92.8°E) in the Xinjang Uygur Autonomous Region. It also deals with several treating methods of chemistry and physics to raise the hatching rate. The main results of the test are as follows. 1. The highest hatching rate(45.01%) is obtained when the cysts are collected directly from brine of the saline lake, the next is cysts of bottom sediments, the lowest is cysts of shore at saline lake. 2. The hatching rate of cysts after shore-term (3months) cold storage (3~4℃) or freezing (about -20℃) is higher (59.33%, 52.51%), cysts immersed in brine and stored at room temperature is the second (49.12%) and dry cysts stored at room temperature is the lowest(31.91%). 3. The hatching rate of cysts after long-term (6 months) immersion in saturated brine of saline lake or sea water in refrigerator (about -20℃) is higher (41.44%, 46.20%), but the hatching rate of cysts immersed in common sea water is poorer (38.01%). 4. The hatching rates of cysts treated with 3% formalin (HCHO) solution after 10 min and 3% potassium permanganate (KMnO4) solution after 20 min are increased by 8.50% and 9.37% respectively higher than that of the control, but with 3% peroxide (H202), it is to no avail. 5. The hatching rate of cysts irradiated with fast neutron (105n/m2·s) after 4~5 min is increased by 8.74~9.55% higher than that of the control. The hatching rate of cysts in cold storage (3~4℃) and freezing storage (about -20℃) are Increased by 15.45% and 8.59% respectively higher than that of the control.