Anabaena flos-aquae is one of the common harmful cyanobacteria species.However, its growth kinetics co-impacted by phosphate concentration, light intensity and temperature have rarely been reported.In this paper, eight PO₄^3--P concentration gradients (0, 0.1, 0.2, 0.5, 0.75, 1.0, 2.5 and 5.0 μmol/L), four water temperatures (15, 20, 25 and 30℃) and four light intensities (1000, 2000, 3000 and 5000 lx) were selected to carry out single factor batch experiments.A maximum specific growth rate (μ_max) was used to indicate algae specific growth rate under optimal growth condition.Half-saturation constant (K_S) could be applied to describe affinity of algae to nutrients.Regression results from Monod model showed that Anabaena flos-aquae reached μ_max at 20℃ and 3000 lx.The μ_max and K_S were 0.447 d^-1 and 0.081 μmol P/L, respectively.Regression results based on Lehman and Steele Model that examines the relationship between specific growth rate and continuous changing of temperature as well as light intensity, demonstrated that the optimum light intensity (I_k) and temperature (T_opt) were 2650.93±88.19 lx and 21.22±0.98℃, respectively.Besides, the kinetic parameters μ_max, K_S, and T_opt from Lehman Model were 0.421±0.011 d^-1, 0.055±0.009 μmol P/L and 21.22±0.98℃, respectively.In the Steele model, μ_max, K_S and I_k were 0.461±0.010 d^-1, 0.051±0.009 μmol P/L and 2650.93±88.19 lx, respectively.