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引用本文:方道艳,施丽梅,李朋富,蔡元锋,张民,史小丽,吴庆龙,于洋.温度对铜绿微囊藻群体培养体系中细菌群落组成及稳定性的影响.湖泊科学,2021,33(6):1660-1674. DOI:10.18307/2021.0605
Fang Daoyan,Shi Limei,Li Pengfu,Cai Yuanfeng,Zhang Min,Shi Xiaoli,Wu Qinglong,Yu Yang.Effects of temperature on composition and stability of bacterial community in colonial Microcystis aeruginosa culture systems. J. Lake Sci.2021,33(6):1660-1674. DOI:10.18307/2021.0605
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温度对铜绿微囊藻群体培养体系中细菌群落组成及稳定性的影响
方道艳1, 施丽梅2, 李朋富1, 蔡元锋3, 张民2, 史小丽2, 吴庆龙2, 于洋2
1.南京大学生命科学学院, 南京 210093;2.中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008;3.中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008
摘要:
微囊藻藻际细菌影响微囊藻的生长及其水华的生消.然而特定微囊藻群体中藻际细菌群落组成对温度变化的响应规律仍不清楚.本文把一株从太湖分离得到的群体铜绿微囊藻,置于不同温度(15、20、25和30℃)下进行培养,分析各培养体系中不同粒径附生或游离细菌群落组成的异同.结果表明:温度显著影响微囊藻群体(>20 μm)附生、单细胞小群体(3~20 μm)附生和游离(0.2~3 μm)细菌群落的组成(PERMANOVA,P<0.01),Sphingomonadales、Pseudomonadales和Cytophagales分别是3组细菌群落中的最优势菌目,相对丰度分别为21.35%、19.74%和33.44%.在3组细菌群落中都存在一些核心优势细菌类群,其丰度相对稳定,对温度变化不敏感.其中在微囊藻群体附生细菌群落中,优势菌属Brevundimonas和OPB56在20~30℃之间培养时其相对丰度较为稳定;单细胞小群体附生细菌群落中的核心优势菌属Mariniradius相对丰度也是在20~30℃之间较为稳定,而Gemmobacter相对丰度在4种温度下均较为一致;游离细菌群落中的核心优势菌属Porphyrobacter相对丰度在20~30℃之间时也相对稳定.另外,在15℃时,单细胞小群体附生和游离细菌群落的多样性都达到最高,总体细菌群落物种相关性网络复杂度最高,但合作性关联最弱.该研究结果对于深入了解微囊藻群体的藻菌关系有重要意义.
关键词:  铜绿微囊藻群体  细菌群落  温度  附生细菌  游离细菌  太湖
DOI:10.18307/2021.0605
分类号:
基金项目:国家水体污染控制与治理科技重大专项(2018ZX07208-004)和国家自然科学基金项目(31971449,31370509)联合资助.
Effects of temperature on composition and stability of bacterial community in colonial Microcystis aeruginosa culture systems
Fang Daoyan1, Shi Limei2, Li Pengfu1, Cai Yuanfeng3, Zhang Min2, Shi Xiaoli2, Wu Qinglong2, Yu Yang2
1.School of Life Sciences, Nanjing University, Nanjing 210093, P. R. China;2.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China;3.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, P. R. China
Abstract:
Bacteria in phycosphere of Microcystis affect growth, development and decline of Microcystis blooms. In specific Microcystis colony, however, the pattern of bacterial community composition in response to different temperatures are still unclear. In this study, a colonial Microcystis aeruginosa strain isolated from Lake Taihu was cultivated at different temperatures (15, 20, 25 and 30℃) to analyse the similarities and differences in the composition of different particle attached or free living bacterial communities in each culture system. Temperature significantly affected community compositions of bacteria including Microcystis colony (>20 μm) attached bacteria, single cell-small colony (3-20 μm) attached bacteria and free-living (0.2-3 μm) bacteria (PERMANOVA,P<0.01), Sphingomonadales, Pseudomonadales and Cytophagales were the most dominant order in the three groups, accounting for 21.35%, 19.74% and 33.44% of the total relative abundance, respectively. There were some core dominant bacterial species in the three groups of bacterial communities, which were relatively stable in abundance and insensitive to temperature changes. The relative abundance of Brevundimonas and OPB56, the dominant genus among the core bacterial community attached with Microcystis colony, were relatively stable when temperature changed from 20 to 30℃. The relative abundance of Mariniradius, the dominant genus among the core bacteria attached with single cell-small colony, was also relatively stable when temperature changed from 20 to 30℃, and the relative abundance of Gemmobacter, the another dominant genus among the core bacteria attached with single cell-small colony, was relatively stable in response to temperature changes. The relative abundance of Porphyrobacter, the dominant genus among the core bacterial community in free-living bacteria, were also relatively stable when temperature changed from 20 to 30℃. Compared with the situations at other three higher temperatures, the complexity of species correlation network at 15℃ was the highest but the cooperative correlation was the weakest. The results of this study were of great significance for understanding the algal-bacterial relationships in phycosphere of colonial Microcystis.
Key words:  Microcystis aeruginosa colony  bacterial community  temperature  attached bacteria  free-living bacteria  Lake Taihu
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