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引用本文:章婷曦,卢小然,魏超,李德芳,秦梦瑶,张利民.太湖悬浮颗粒物细菌碱性磷酸酶编码基因的分布特征.湖泊科学,2019,31(5):1368-1378. DOI:
ZHANG Tingxi,LU Xiaoran,WEI Chao,LI Defang,QIN Mengyao,ZHANG Limin.Spatial and temporal distribution of alkaline phosphatase encoding genes in suspended particulates in Lake Taihu. J. Lake Sci.2019,31(5):1368-1378. DOI:
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太湖悬浮颗粒物细菌碱性磷酸酶编码基因的分布特征
章婷曦1,2,3, 卢小然1, 魏超1, 李德芳1, 秦梦瑶1, 张利民1
1.南京师范大学环境学院, 南京 210023;2.江苏省地理信息资源开发与利用协同创新中心, 南京 210023;3.江苏省物质循环与污染控制实验室, 南京 210023
摘要:
颗粒态有机磷的碱性磷酸酶矿化是水生态系统磷循环的重要环节,对细菌碱性磷酸酶编码基因的解析有利于揭示浅水富营养化湖泊有机磷矿化的微生物驱动机制.本文以太湖不同生态类型湖区悬浮颗粒物为研究对象,运用荧光定量PCR技术,探究太湖水体中悬浮颗粒物细菌碱性磷酸酶phoXphoD基因的时空分布特征,以及影响两类基因丰度的主要环境因子.结果表明:太湖不同生态类型湖区中phoD基因丰度是phoX基因的6~42倍,且二者均存在显著的时空分布差异.6月,河口区phoX(9.18×104 copies/L)和phoD(1.88×106 copies/L)基因丰度均最高,其次分别为草型区、湖心区和藻型区.与6月相比,9月各湖区phoD基因丰度显著降低,而phoX基因丰度在藻型区和草型区则有所升高.9月,草型区phoX基因丰度最高(5.70×104 copies/L),河口区最低(1.49×104 copies/L).水生植物对phoXphoD基因丰度具有重要贡献.悬浮颗粒物细菌phoX基因丰度可能被低估.溶解氧、总氮和总磷是影响太湖phoXphoD基因丰度的主要环境因子.
关键词:  碱性磷酸酶  编码基因  悬浮颗粒物  太湖  qPCR
DOI:
分类号:
基金项目:国家水体污染控制与治理科技重大专项(2017ZX07202-004)和国家自然科学基金项目(41303058,41877336)联合资助.
Spatial and temporal distribution of alkaline phosphatase encoding genes in suspended particulates in Lake Taihu
ZHANG Tingxi1,2,3, LU Xiaoran1, WEI Chao1, LI Defang1, QIN Mengyao1, ZHANG Limin1
1.School of Environment, Nanjing Normal University, Nanjing 210023, P. R. China;2.Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, P. R. China;3.Jiangsu Key Laboratory of Material Cycle & Pollution Control, Nanjing 210023, P. R. China
Abstract:
The mineralization of organic P to orthophosphate in suspended particles by alkaline phosphatase plays an important role in phosphorus cycling in aquatic ecosystem, especially in shallow lake. The study on bacterial alkaline phosphatase-encoding genes is of great significance to reveal the microbial driving mechanisms of organic P mineralization. We identified the spatial and temporal distribution characteristics of the abundance of phoX and phoD genes in suspended particles in four ecological regions in Lake Taihu, a large shallow eutrophic lake,and explored the main environmental factors affecting the abundance of the two genes. The qPCR analysis suggested that phoD gene abundance was 6-42 times higher than phoX gene abundance in four ecological regions in the study period, and both of phoD and phoX genes abundance in suspended particles has significant difference in spatial and temporal distribution. The highest abundance of phoX (9.18×104 copies/L) and phoD (1.88×106 copies/L) were found in the river estuaries in June, followed by that in macrophyte-dominated zone, central lake zone and algae-dominated zone. Compared with the genes abundances in June, the abundance of phoD gene in four ecological regions decreased significantly in September while phoX gene abundance increased in algae-dominated zone and macrophyte-dominated zone. In September, the abundance of phoX gene (5.70×104 copies/L) in macrophyte-dominated zone was the highest and the lowest was in the river estuaries (1.49×104 copies/L). Macrophytes play an important role in determining the abundance of phoD and phoX genes. The abundance of phoX genes in suspended particles may be underestimated. Dissolved oxygen, total nitrogen and total phosphorus are the main environmental factors affecting the abundance of bacterial phoX and phoD genes in suspended particulates.
Key words:  Alkaline phosphatase  encoding gene  suspended particles  Lake Taihu  qPCR
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