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引用本文:周英萍,唐美珍,曹彦雪,周高洁,陈娟娟,马文秀.一株耐冷异养硝化菌的分离鉴定及脱氮特性.湖泊科学,2019,31(3):746-754. DOI:10.18307/2019.0313
ZHOU Yingping,TANG Meizhen,CAO Yanxue,ZHOU Gaojie,CHEN Juanjuan,MA Wenxiu.Isolation and denitrification characteristics of a psychrotrophic and heterotrophic nitrification bacterium. J. Lake Sci.2019,31(3):746-754. DOI:10.18307/2019.0313
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一株耐冷异养硝化菌的分离鉴定及脱氮特性
周英萍, 唐美珍, 曹彦雪, 周高洁, 陈娟娟, 马文秀
曲阜师范大学南四湖湿地生态与环境保护重点实验室, 曲阜 273165
摘要:
利用异养硝化培养基,冬季从南四湖人工湿地底泥中分离筛选到一株具有较高脱氮性能的菌株Z01.经形态特征、生理生化特性以及16S rDNA序列分析,初步鉴定该菌株为假单胞菌属(Pseudomonas sp.),命名为Pseudomonas sp.Z01.实验探究得到菌株Z01的最佳初始投菌量为7%,最适温度、pH和盐度分别为15℃、6.0~8.0和0.3%,最佳碳源为葡萄糖.在最适生长条件下,通过氨氮去除试验发现该菌株对模拟污水中氨氮的去除率有一定提高,是异养硝化培养基中氨氮去除效率的1.1倍.此外,该菌株对模拟废水中总氮和COD也具有较好的去除效果.研究表明,菌株Z01在冬季污水处理中硝化脱氮具有良好的应用前景和很大的发展空间.
关键词:  低温异养硝化  生物脱氮  菌株  氨氮  南四湖
DOI:10.18307/2019.0313
分类号:
基金项目:2017年曲阜师范大学国家级大学生创新创业训练计划项目(201710446074)和国家自然科学基金项目(31700433,31672314)联合资助.
Isolation and denitrification characteristics of a psychrotrophic and heterotrophic nitrification bacterium
ZHOU Yingping, TANG Meizhen, CAO Yanxue, ZHOU Gaojie, CHEN Juanjuan, MA Wenxiu
Key Laboratory of Nansi Lake Wetland Ecosystem & Environment Protection, Qufu Normal University, Qufu 273165, P.R.China
Abstract:
A heterotrophic nitrifying bacteria strain Z01 was isolated from the sediment of Lake Nansi constructed wetland in winter, and it was studied for its capacity of nitrification and nitrogen removal. Methods of morphological, physiological and biochemical were used to identify strain Z01 as well as 16S rDNA analysis. It is affiliated to be Pseudomonas sp., which named Pseudomonas sp. Z01. These data showed that the optimum dosage, temperature, pH and salinity of microbial strains Z01 were 7%, 15℃, 6.0-8.0 and 0.3%, as sucrose as optimum carbon. Under the optimum removal efficiency of ammonia nitrogen from simulated wastewater by strain 10 was 1.1 times higher than that of heterotrophic nitrification medium. Moreover, it was better on the removal performance of the total nitrogen and COD in the simulated wastewater. Therefore, strain Z01 has a good application prospect and great development space in nitrification denitrification of sewage treatment in winter.
Key words:  Low temperature heterotrophic nitrification  biological denitrification  strain  ammonia nitrogen  Lake Nansi
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