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引用本文:王捷,石瑛,刘琪,李砧,张猛,谢树莲.汾河太原河段水华藻类分类及分子系统研究.湖泊科学,2018,30(5):1332-1342. DOI:10.18307/2018.0515
WANG Jie,SHI Ying,LIU Qi,LI Zhen,ZHANG Meng,XIE Shulian.Taxonomic and molecular phylogenetics of bloom-forming algae from the Taiyuan section of Fenhe River, China. J. Lake Sci.2018,30(5):1332-1342. DOI:10.18307/2018.0515
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汾河太原河段水华藻类分类及分子系统研究
王捷1,2, 石瑛1, 刘琪2, 李砧1, 张猛1, 谢树莲2
1.太原师范学院生物系, 太原 030032;2.山西大学生命科学学院, 太原 030006
摘要:
2012-2016年,每年的春、夏、秋季对汾河太原河段进行浮游植物样品采集.通过对样品的形态观察和描述,共鉴定出5种水华优势种,5月发生的裸藻水华优势种为裸藻属的膝曲裸藻(Euglena geniculata)和血红裸藻(E.sanguinea).而7-9月发生的微囊藻水华优势种为微囊藻属的铜绿微囊藻(Microcystis aeruginosa)、挪氏微囊藻(M.novacekii)和惠氏微囊藻(M.wesenbergii).分离纯化共得到11株单克隆水华藻,其中铜绿微囊藻8株,挪氏微囊藻2株,血红裸藻1株.运用cpcBA-IGS、gyrB和cpSSU基因序列构建分子系统发育树,进一步确定水华藻的系统分类地位,结果表明cpcBA-IGS是研究汾河太原河段铜绿微囊藻分类很好的分子标记,而cpSSU基因可很好地区分血红裸藻和其他裸藻种.
关键词:  汾河太原段  水华  微囊藻  裸藻  形态分类学  系统发育
DOI:10.18307/2018.0515
分类号:
基金项目:山西省重点研发计划项目(201603D321001,201603D321008)、山西省藻类植物标本资源及大型淡水绿藻培养开发平台建设项目(2015091004-0102)和山西省高等学校科技创新项目(2013145)联合资助.
Taxonomic and molecular phylogenetics of bloom-forming algae from the Taiyuan section of Fenhe River, China
WANG Jie1,2, SHI Ying1, LIU Qi2, LI Zhen1, ZHANG Meng1, XIE Shulian2
1.Department of Biology, Taiyuan Normal University, Taiyuan 030032, P. R. China;2.School of Life Science, Shanxi University, Taiyuan 030006, P. R. China
Abstract:
From 2012 to 2016, phytoplankton samples were collected from the Taiyuan section of Fenhe River in the spring, summer and autumn. In this study, a total of five dominant species were identified and morphologically described. Water bloom of Euglena can be found in May, and the dominant species were E. geniculata and E. sanguinea. From July to September, the dominant species became different kinds of Microcystis in different sample sites, including M. aeruginosa, M. novacekii and M. wesenbergii, respectively. A total of eleven strains of bloom-forming algae were successfully isolated. Among them, there were eight M. aeruginosa strains, two M. novacekii strains and one Euglena sanguinea strain. To study the taxonomic status of bloom-forming algae in the Taiyuan section of Fenhe River, phylogenetic analyses based on cpcBA-IGS, gyrB and cpSSU genes were performed from ten isolated strains of Microcystis and one E. sanguinea, respectively. The results indicated that cpcBA-IGS may be a good molecular marker in studying the taxonomy of M. aeruginosa in Fenhe River, and phylogenetic analysis based on cpSSU gene was thought to be effective for understanding relationships among E. sanguinea and the other Euglena species.
Key words:  Taiyuan section of Fenhe River  water bloom  Microcystis  Euglena  diversity  morphology  phylogeny
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