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引用本文:黄代中,肖文娟,刘云兵,刘竟远,周易勇.浅水湖泊沉积物脱氢酶活性的测定及其生态学意义.湖泊科学,2009,21(3):345-350. DOI:10.18307/2009.0305
HUANG Daizhong,XIAO Wenjuan,LIU Yunbing,LIU Jingyuan,ZHOU Yiyong.Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance. J. Lake Sci.2009,21(3):345-350. DOI:10.18307/2009.0305
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浅水湖泊沉积物脱氢酶活性的测定及其生态学意义
黄代中1,2, 肖文娟1,2, 刘云兵1,2, 刘竟远1, 周易勇1
1.中国科学院水生生物研究所, 武汉 430072;2.中国科学院研究生院, 北京 100049
摘要:
以武汉市浅水湖泊(月湖)为实验对象确定了沉积物脱氢酶活性(DHA)测定的最佳条件:以经甲醛灭菌的沉积物为对照,以氯化三苯基四氮唑为电子受体(0.4%),沉积物用量为0.5g,pH值为7.5,培养时间为3h.据上述条件测定的DHA比未优化条件下的相应值高3-5倍,且表现出与沉积物有机质含量更为显著的正相关关系.武汉东湖3个子湖共23个采样点沉积物样品的测试结果进一步证实了上述方法的可行性,同时初步揭示了湖泊沉积物脱氢酶反映微生物活性与有机质状态的生态学意义.
关键词:  沉积物  脱氢酶  TTC  微生物活性  有机质  湖泊
DOI:10.18307/2009.0305
分类号:
基金项目:国家“973”计划(2008CB418005);中国科学院知识创新工程重要方向项目(KZCX2-YW-426-01);中国科学院知识创新工程重大交叉项目(KZCX1-YW-14-1)
Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance
HUANG Daizhong1,2, XIAO Wenjuan1,2, LIU Yunbing1,2, LIU Jingyuan1, ZHOU Yiyong1
1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China;2.Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China
Abstract:
The samples were taken from the shallow urban lakes(Lake Yuehu) in Wuhan city to optimize the determination of dehydrogenase activity (DHA) in sediment, which gave the following suitable conditions: the sediment sterilized by formaldehyde was used as the control, the concentration of electron acceptor (2,3,5-triphenyl-2H-tetrazolium chloride) was 0.4%, the sediment amount used was 0.5g, pH value was 7.5 and the reaction time was 3h. Under these conditions, the DHA assayed was 3-5 times higher, and its positive relation to the content of organic matter in sediment was more significant, compared to those determined by the unmodified method. The data based on the 23 sites from 3 basins of Lake Donghu demonstrated the feasibility of the method. In the same time, the ecological significance of DHA was further shown in terms of the measurements of microbial activities and organic matter status in lake sediment
Key words:  Sediment  dehydrogenase  TTC  microbial activities  organic matter  lake
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