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引用本文:陆源,文建凡,吕天雯.滇池铜绿微囊藻(M. aeruginosa Kutz)的分离培养与总DNA提取的改进.湖泊科学,2001,13(3):285-288. DOI:10.18307/20010314
LU Yuan,WEN Jianfan,LV Tianwen.Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kutz in Dianchi Lake. J. Lake Sci.2001,13(3):285-288. DOI:10.18307/20010314
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滇池铜绿微囊藻(M. aeruginosa Kutz)的分离培养与总DNA提取的改进
陆源, 文建凡, 吕天雯
中国科学院昆明动物研究所细胞与分子进化开放实验室, 昆明 650223
摘要:
利用稀释法结合平板分离培养法将采自滇池水华中的铜绿微囊藻进行了成功的分离纯化.对比纯化培养前后,发现纯化后该藻的生长速度明显加快,密度大为提高,约为纯化前的5倍.用乙醇乙醚混合液对该藻作预处理后,破坏了其胶质被,再经常规方法提取总DNA,其得率提高3-4倍.
关键词:  铜绿微囊藻  分离培养  总DNA提取
DOI:10.18307/20010314
分类号:
基金项目:云南省中青年学术和技术带头人培养经费资助;云南省应用基础研究基金联合资助
Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kutz in Dianchi Lake
LU Yuan, WEN Jianfan, LV Tianwen
Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China
Abstract:
M. aeruginosa Kutz is harmful to ecological environment of lakes.The algae in water bloom of Dianchi Lake was isolated with methods of dilution and agar solid-media culture.It was found that after purification, grow thing rate of the algae was raised by 5 times in liquid culture media.When extracting total DNA of the algae, if the cells were pre-treated by alcohol + ether (1:1), their gelatinous sheathes were broken and the production of total DNA increased by 3-4 times.The improvement of DNA ex traction is useful to molecular biology studies of the algae.
Key words:  M. aeruginosa Kutz  pure cultivation  total DNA extraction
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