%0 Journal Article %T 小麦麸皮水浸提液对铜绿微囊藻(Microcystis aeruginosa)光合色素含量和叶绿素荧光诱导动力学的影响 %T Effect of wheat bran leachate on the photosynthetic pigments contents and chlorophyll fluorescence induction dynamics of Microcystis aeruginosa %A 李藩 %A 李仁辉 %A 于晓章 %A 邵继海 %A LI,Fan %A LI,Renhui %A YU,Xiaozhang %A SHAO,Jihai %J 湖泊科学 %J Journal of Lake Sciences %@ 1003-5427 %V 25 %N 3 %D 2013 %P 373-377 %K 小麦麸皮;水浸提液;铜绿微囊藻;细胞色素;叶绿素荧光 %K Wheat bran;leachate;Microcystis aeruginosa;pigments;chlorophyll fluorescence %X 小麦麸皮水浸提液(WBL)对铜绿微囊藻(Microcystis aeruginosa)具有较强的抑制作用.为了阐明铜绿微囊藻光合系统对WBL胁迫的响应,本文分析了WBL胁迫下M.aeruginosa CHAB-109细胞光合色素含量及叶绿素荧光诱导动力学变化.结果显示,当WBL浓度为1.6和3.2 g/L时,M.aeruginosa CHAB-109单位细胞内Chl.a和类胡萝卜素含量均显著低于对照.Chl.a光诱导荧光动力学分析结果显示,WBL胁迫下单位反应中心吸收的光能(ABS/RC)、单位反应中心捕获的用于还原QA的能量(TR0/RC)及单位反应中心捕获的用于电子传递的能量(ET0/RC)均受到显著抑制.光合系统Ⅱ(PSⅡ)能量分配比率参数分析结果显示,WBL对光合系统反应中心电子供体侧没有影响,但是显著抑制光合系统反应中心电子受体侧电子传递.综合分析WBL诱导下M.aeruginosa CHAB-109 PSⅡ能量流动比活性能参数和能量分配比率参数显示,反应中心电子受体侧电子由反应中心传递到QAQA更远的部位均为WBL的抑制作用位点. %X Previous study indicated that wheat bran leachate(WBL) exhibited strong inhibitory effect against Microcystis aeruginosa.In order to elucidate the responses of M.aeruginosa photosystem to the stress of WBL,pigments and chlorophyll fluorescence transient of M.aeruginosa CHAB-109 under the stress of WBL were analyzed.The results indicated that intracellular pigment contents decreased when WBL was of 1.6 and 3.2 g/L.Results of chlorophyll fluorescence transient analysis indicated that the absorbed energy(ABS/RC),the trapped energy for reduce of QA (TR0/RC),the trapped energy for electron transport(ET0/RC) were all inhibited by WBL.Energy flux ratio analysis of PSⅡ indicated that no damage induced by WBL at the electrons providing side of reaction centre of PSⅡ,but it can damage the acceptor side of reaction centre.Combining the results of energy fluxes and flux ratios indicated that the electron transport chain from reaction centre to QA and far beyond QA are the damage sits of WBL on Microcystis aeruginosa. %R 10.18307/2013.0310 %U http://www.jlakes.org/ch/reader/view_abstract.aspx %1 JIS Version 3.0.0